Manual Pair Formation and Electrofusion of Protoplasts Using Platinum Microelectrodes
نویسندگان
چکیده
منابع مشابه
Effects of microgravitation on electrofusion of plant cell protoplasts.
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15 صفحه اولBehavior and viability of tobacco protoplasts in response to electrofusion parameters.
This investigation examines responses of protoplasts in a systematic and quantitative way to the various electrical treatments used to achieve electrofusion and their individual and cumulative effect on protoplast viability. Mesophyll and cell suspension protoplasts from two species of the same genera, Nicotiana tabacum and N. rustica var brasilia were used in these experiments. Optimal frequen...
متن کاملA simple method for large-scale electrofusion and culture of plant protoplasts.
A simple method is described for the electrofusion of plant protoplasts. Protoplasts were aggregated in a radio-frequency field (10 V RMS, 0.5 MHz) for 15-30 s with an inter-electrode distance of 5 mm. They were then fused with a 300-V DC pulse. The protoplasts were able to divide after this treatment. A transferrable electrode permitted electrofusion of 1-ml volumes of culture in standard tiss...
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ژورنال
عنوان ژورنال: Plant tissue culture letters
سال: 1988
ISSN: 1884-7706,0289-5773
DOI: 10.5511/plantbiotechnology1984.5.37